|
Protoplasts were enzymatically isolated from suspension cltures of sweet potato. High yields of single protoplasts were produced from nonembryogenic cell aggregates. However, most protoplasts obtained from embryogenic cell clumps were spontaneously fused during enzyme treatment; a small portion of them remained single. Upon transfer to Murashige and Skoog´s(MS) liquid medium supplemented with 0.1 ㎎/l 6-benzyladenine(BA) and 1 ㎎/l 2,4-dichlorophenoxyacetic acid(2,4-D), protoplasts from nonembryogenic cell aggregates sustained cell divisions to form callus. Upon subculture onto MS media with 0.2 ㎎/l 2,4-D or without growth regulators, the callus did not give rise to any organs. On the other hand, first cell division of single protoplasts from embryogenic cell clumps was sporadically observed.
|